Journal: Nature communications

Article Title: Quantification of ligand and mutation-induced bias in EGFR phosphorylation in direct response to ligand binding.

doi: 10.1038/s41467-023-42926-8

Figure Lengend Snippet: Fig. 2 | Ligand bias for WT EGFR. A Raw dose response curves for Y1068 and Y1173 phosphorylation per EGFR molecule, for the ligands EGF, TGFα, and epiregulin. Each point represents the ratio of either anti-pY1068 or anti-pY1173 fluorescence and EGFR-mTurqfluorescence for one individual vesicle. Each curve contains ~1000 to ~3000 data points (single vesicles). B Bias plots. Shown are means (symbols) and standard errors (often smaller than symbols). The epiregulin points diverge from

Article Snippet: The ligands used in this study were EGF (8916sf, Cell Signaling), TGFα (239A100, R&D Systems), Epiregulin (1195EP025 CF, R&D Systems), and EGF-tetramethylrhodamine (E3481, Thermofisher).

Techniques: Phospho-proteomics

Journal: Nature communications

Article Title: Quantification of ligand and mutation-induced bias in EGFR phosphorylation in direct response to ligand binding.

doi: 10.1038/s41467-023-42926-8

Figure Lengend Snippet: Fig. 3 | Ligand bias in L834R EGFR phosphorylation. A Single vesicle dose response curves for Y1068 and Y1173 phosphorylation per EGFR molecule, for EGF, TGFα, and epiregulin. Each point represents the ratio of either anti-pY1068 or anti- pY1173 fluorescence and EGFR-mTurq fluorescence for one individual vesicle. Each curve contains ~1000 to ~1800 data points. B Bias plots. Shown are means

Article Snippet: The ligands used in this study were EGF (8916sf, Cell Signaling), TGFα (239A100, R&D Systems), Epiregulin (1195EP025 CF, R&D Systems), and EGF-tetramethylrhodamine (E3481, Thermofisher).

Techniques: Phospho-proteomics

Journal: Biology of reproduction

Article Title: Effect of epidermal growth factor-like peptides on the metabolism of in vitro- matured mouse oocytes and cumulus cells.

doi: 10.1095/biolreprod.113.115311

Figure Lengend Snippet: FIG. 3. Effect of FSH, EGF, and EGF-like peptides on COC b-O-linked glycosylation. A1) Protein b-O-linked glycosylation was examined at 12 h IVM in the presence of control (no treatment), FSH, AREG, EREG, BTC, or EGF and b-O-linked glycosylation (CTD110.6) and nuclear staining (PI) fluorescence were imaged. Images shown are representative of 30 COCs per treatment group over three replicate experiments. Original magnification 360. A2) Quantification of relative CTD110.6 fluorescence in cumulus cells and oocytes. B) COC mRNA expression of Ogt was measured at 6 h IVM (n ¼ 6). Bars not sharing a common letter are significantly different (P , 0.02). The data represent means 6 SEM.

Article Snippet: COC IVM IVM COCs were cultured in bicarbonate buffered aMEM (Gibco) supplemented with 3 mg/ml BSA and either recombinant human FSH (50 mIU/ml; Puregon; Organon, Oss, The Netherlands), recombinant human EGF (10 ng/ml; R&D Systems, Minneapolis, MN), recombinant mouse AREG (50 ng/ml; R&D Systems), recombinant mouse EREG (50 ng/ml; R&D Systems), or recombinant mouse BTC (50 ng/ml; R&D Systems), at 378C with 5% CO 2 in air.

Techniques: Glycoproteomics, Control, Staining, Fluorescence, Expressing

Journal: Developmental cell

Article Title: The bone microenvironment increases phenotypic plasticity of ER+ breast cancer cells

doi: 10.1016/j.devcel.2021.03.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ERE-pWPXL/GFP , This paper (modified from Plasmid # 12257; gift from Didier Trono to addgene) EPFL , N/A.

Techniques: Recombinant, Transfection, Sequencing, Negative Control, Luciferase, Plasmid Preparation, Modification, Software